Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

Ikeda, Masaki

doi:https://doi.org/10.1186/s12866-015-0600-8

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Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

http://hdl.handle.net/10271/3140

名前 / ファイル	ライセンス	アクション
論文本文 (5.4 MB)

Item type

学位論文 / Thesis or Dissertation(1)

公開日

2017-01-28

タイトル

Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

言語

eng

キーワード

主題

Haemophilus influenzae

キーワード

主題

NTHi

キーワード

主題

Intracellular invasion

キーワード

主題

Protein-E

キーワード

主題

Vitronectin

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_db06

資源タイプ

doctoral thesis

アクセス権

open access

アクセス権URI

http://purl.org/coar/access_right/c_abf2

その他のタイトル

無莢膜型ヘモフィルスインフルエンザ菌は気管支上皮細胞への接着と侵入にProtein-Eとビトロネクチンの相互作用を利用する

著者

Ikeda, Masaki

書誌情報

en : BMC Microbiology

巻 15, p. 263, 発行日 2015-11-14

出版者

BioMed Central

言語

抄録

内容記述タイプ

Abstract

内容記述

Background: Nontypeable Haemophilus influenzae (NTHi) is one of the most common Gram-negative pathogens in otitis media and exacerbation of chronic obstructive pulmonary disease. NTHi has been reported to invade bronchial epithelial cells. This penetration enables NTHi to evade the host immune system and antibiotics, and it seems to be related to the intractable features of these diseases. However, the precise mechanism of the invasion has been unknown. We hypothesized that protein-E, an outer membrane protein of NTHi, plays a role in this penetration into bronchial epithelial cells.
Results: We utilized two NTHi strains. NTHi efficiently attached to plate-bound vitronectin (254-309 / field at 1,000× magnification) and this attachment was blocked by pretreatment with protein-E peptide (PE84-108). The blockade of adhesion was dependent on the concentration of PE84-108. NTHi strains invaded bronchial epithelial cells and the intracellular bacteria were localized in early endosomes. Furthermore, intracellular invasion of NTHi was also blocked by PE84-108, but not by Arg-Gly-Asp (RGD) peptide. Pretreatment with PE84-108 significantly prevented cells from being invaded by both NTHi strains, which was confirmed by fluorescent microscope observation. In addition, pretreatment with PE84-108 significantly reduced percentages of CFU after gentamicin treatment of cells per input CFU.
Conclusions: These results suggest that NTHi does not directly bind to the cell surface, but binds to host vitronectin that is bound to the cell surface, via bacterial protein-E. Bacterial protein-E and host vitronectin play a role in the attachment to bronchial epithelial cells and is also involved in the subsequent intracellular invasion of NTHi. A novel vaccine or treatment strategy targeting the protein-E-vitronectin axis may prevent respiratory intracellular infection of NTHi and may lead to better clinical outcomes.

言語

学位名

博士（医学）

学位の区分

内容記述

doctoral

学位の分野

内容記述

医学系研究科

学位授与機関

学位授与機関識別子Scheme

kakenhi

学位授与機関識別子

13802

学位授与機関名

浜松医科大学

学位授与年月日

2016-03-14

学位授与番号

甲第718号

EISSN

収録物識別子タイプ

EISSN

収録物識別子

1471-2180

PubMed番号

識別子タイプ

PMID

Versions

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2023-06-20 16:52:24.594962

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Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells

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