Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells

Nagahashi, Kotomi

doi:https://doi.org/10.1007/s10616-016-0059-y

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Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells

http://hdl.handle.net/10271/3214

名前 / ファイル	ライセンス	アクション
論文本文 (446.0 kB)

Item type

学位論文 / Thesis or Dissertation(1)

公開日

2018-02-07

タイトル

Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells

言語

eng

キーワード

主題

vitamin K

キーワード

主題

キーワード

主題

Drosophila

キーワード

主題

coagulation factor

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_db06

資源タイプ

doctoral thesis

アクセス権

open access

アクセス権URI

http://purl.org/coar/access_right/c_abf2

その他のタイトル

ショウジョウバエシュナイダーS2細胞における哺乳動物γ-グルタミルカルボキシラーゼの共発現およびvit.Kサイクルの修飾による活性化ヒト凝固第VII因子合成の成功

著者

Nagahashi, Kotomi

書誌情報

en : Cytotechnology

巻 69, 号 2, p. 317-327, 発行日 2017-01-09

出版者

Springer Nature

言語

抄録

内容記述タイプ

Abstract

内容記述

Mammalian gamma-glutamyl carboxylase and reduced vitamin K are indispensable for synthesis of mature mammalian vitamin K dependent proteins including some of blood coagulation factors (factors II, VII, IX, and X). It was well known that Drosophila melanogaster expressed gamma-glutamyl carboxylase and possessed a vit.K cycle although native substrates for them have not been identified yet. Despite the potential capability of gamma calboxylation in Drosophila melanogaster derived cells such as S2 cells, Drosophila gamma-glutamyl carboxylase failed to gamma carboxylate a peptide fused to the human coagulation factor IX propeptide. Thus, it had been believed that the Drosophila system was not adequate to synthesize mammalian vit.K dependent proteins. Indeed, we previously attempted to synthesize biologically active factor VII in S2 cells although we were not able to obtain it. However, recently, a successful transient expression of biologically active human factor IX from S2 cells were reported. In the present study, several expression vectors which enables to express mammalian GGCX, VKORC1, and/or PDIA2 along with F7 were developed. S2 cells transfected with pMKA85, pMAK86, and pMAK219 successfully synthesized active FVII. Thus, mammalian GGCX was indispensable to synthesize active FVII while mammalian VKORC1 and PDIA2 were not critical but supportive factors for S2 cells.

言語

学位名

博士（医学）

学位の区分

内容記述

doctoral

学位の分野

内容記述

医学系研究科

学位授与機関

学位授与機関識別子Scheme

kakenhi

学位授与機関識別子

13802

学位授与機関名

浜松医科大学

学位授与年月日

2017-02-09

学位授与番号

甲第740号

ISSN

収録物識別子タイプ

PISSN

収録物識別子

0920-9069

EISSN

収録物識別子タイプ

EISSN

収録物識別子

1573-0778

PubMed番号

識別子タイプ

PMID

Versions

Ver.1

2023-06-20 16:50:23.482853

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Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells

× Nagahashi, Kotomi

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