| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-04-26 |
| タイトル |
|
|
タイトル |
Overexpression of Sal-like protein 4 in head and neck cancer: epigenetic effects and clinical correlations |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
HNSCC |
| キーワード |
|
|
主題 |
SALL4 |
| キーワード |
|
|
主題 |
Biomarker |
| キーワード |
|
|
主題 |
Epigenetic regulation |
| キーワード |
|
|
主題 |
DNMT3A |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Misawa, Kiyoshi
Misawa, Yuki
Mima, Masato
Yamada, Satoshi
Imai, Atsushi
Mochizuki, Daiki
Nakagawa, Takuya
Kurokawa, Tomoya
Endo, Shiori
Kawasaki, Hideya
Brenner, Chad J.
Mineta, Hiroyuki
|
| 書誌情報 |
Cellular Oncology
巻 43,
号 4,
p. 631-641,
発行日 2020-08
|
| 出版者 |
|
|
出版者 |
Springer Nature |
| 権利 |
|
|
権利情報 |
"This is a post-peer-review, pre-copyedit version of an article published in ""Cellular Oncology"". The final authenticated version is available online at: https://doi.org/10.1007/s13402-020-00509-5. " |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Background Sal-like protein 4 (SALL4), an embryonic stem cell factor, has been reported to play an essential role in embryogenesis and oncogenesis. As yet, however, the expression and role of this transcription factor in head and neck squamous cell carcinoma (HNSCC) has not been established.
Methods We assessed SALL4 mRNA expression in a well-characterised dataset of 230 HNSCC samples (test cohort 110 cases and validation cohort 120 cases). We also transfected HNSCC cells (FaDu and UM-SCC-6) with SALL4 siRNA and assessed its effects on proliferation and expression of specific epigenetic factors in order to uncover the role of SALL4 in HNSCC.
Results Overexpression of SALL4 was detected in tumour samples of both cohorts. HNSCC cells treated with SALL4 siRNA showed a reduction in growth and a decrease in DNA methyltransferase 3 alpha (DNMT3A) expression. In the patient cohorts, SALL4 overexpression was found to significantly correlate with disease recurrence (p<0.001) and SALL4 methylation status (p=0.002). We also found that DNMT3A was significantly upregulated upon SALL4 upregulation (p<0.001). High expression levels of SALL4 correlated with decreases in disease?free survival (DFS) rates (log?rank test, p<0.001). Multivariate analysis revealed that SALL4 expression served as an independent prognostic factor for DFS (hazard ratio: 2.566, 95% confidence interval: 1.598?4.121? p<0.001).
Conclusions Our findings indicate that SALL4 upregulation correlates with HNSCC tumour aggressiveness and an adverse patient outcome. Our findings also indicate that DNMT3A may synergistically contribute to the regulatory effects of SALL4. Our findings provide insight into SALL4-mediated HNSCC development via epigenetic modulation. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2211-3428 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2211-3436 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
32240499 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s13402-020-00509-5 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
Cell Oncol-43-631.pdf (5.0 MB)
