Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis

馬屋原, 孝恒

doi:https://doi.org/10.1111/cea.13672

WEKO3

lat lon distance

[[sub_check.contents]]

[[sub_radio.contents]]

Field does not validate

[[sub_attr.contents]]　

インデックスツリー

アイテム

Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis

http://hdl.handle.net/10271/00003857

名前 / ファイル	ライセンス	アクション
論文本文 (1.4 MB)

Item type

学位論文 / Thesis or Dissertation(1)

公開日

2021-07-01

タイトル

Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis

言語

eng

キーワード

主題

atopic dermatitis

キーワード

主題

galectin-7

キーワード

主題

alarmin

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_db06

資源タイプ

doctoral thesis

アクセス権

open access

アクセス権URI

http://purl.org/coar/access_right/c_abf2

その他のタイトル

アトピー性皮膚炎における皮膚バリア障害に対するガレクチン-7の保護的役割

著者

馬屋原, 孝恒

書誌情報

en : Clinical & Experimental Allergy

巻 50, 号 8, p. 922-931, 発行日 2020-08

出版者

John Wiley and Sons

言語

権利

言語

権利情報

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Copyright 2020 The Authors. Clinical & Experimental Allergy published by John Wiley & Sons Ltd

抄録

内容記述タイプ

Abstract

内容記述

Background: Atopic dermatitis (AD) patients have a barrier disorder in association with Th2 dominant skin inflammation. Galectin-7 (Gal-7), a soluble unglycosylated lectin, is highly expressed in the stratum corneum of AD patients. However, the biological significance of increased Gal-7 expression in AD skin lesions remains unclear.
Objective: We aimed to investigate the production mechanism and functional role of Gal-7 in AD patients and IL-4/IL-13-stimulated epidermal keratinocytes.
Methods: We assessed the Gal-7 expression levels in skin lesions and sera from AD patients. Gal-7 levels were also measured in monolayered normal human epidermal keratinocytes (NHEKs) and 3-dimensional (3D)-reconstructed epidermis in the presence or absence of IL-4/IL-13 with or without Stat3, Stat6 or Gal-7 gene silencing.
Results: Gal-7 was highly expressed in the stratum corneum or intercellular space of AD lesional epidermis as assessed by the stratum corneum proteome analysis and immunohistochemistry. A positive correlation was noted between serum Gal-7 level and transepidermal water loss in patients with AD. These clinical findings were corroborated by our in vitro data, which showed that IL-4/IL-13 facilitated the extracellular release of endogenous Gal-7 in both monolayered NHEKs and 3D-reconstructed epidermis. This machinery was caused by IL-4/IL-13-induced cell damage and inhibited by knockdown of Stat6 but not Stat3 in NHEKs. Moreover, we performed Gal-7 knockdown experiment on 3D-reconstructed epidermis and the result suggested that endogenous Gal-7 serves as a protector from IL-4/IL-13-induced disruption of cell-to-cell adhesion and/or cell-to-extracellular matrix adhesion.
Conclusion and Clinical Relevance: Our study unveils the characteristic of Gal-7 and its possible role as an alarmin that reflects the IL-4/IL-13-induced skin barrier impairment in AD.

言語

学位名

博士（医学）

学位の区分

内容記述

doctoral

学位の分野

内容記述

医学系研究科

学位授与機関

学位授与機関識別子Scheme

kakenhi

学位授与機関識別子

13802

学位授与機関名

浜松医科大学

学位授与年月日

2020-12-11

学位授与番号

甲第850号

ISSN

収録物識別子タイプ

PISSN

収録物識別子

0954-7894

EISSN

収録物識別子タイプ

EISSN

収録物識別子

1365-2222

PubMed番号

識別子タイプ

PMID

Versions

Ver.1

2023-06-20 16:26:32.426362

Show All versions

Cite as

エクスポート

OAI-PMH

JPCOAR 2.0
JPCOAR 1.0
DublinCore
DDI

Other Formats

JSON
BIBTEX

インデックスリンク

インデックスツリー

アイテム

Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis

× 馬屋原, 孝恒

Versions

Share

Cite as

エクスポート