| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-10-01 |
| タイトル |
|
|
タイトル |
Postmortem distribution/redistribution of buformin in body fluids and solid tissues in an autopsy case using liquid chromatography?tandem mass spectrometry with QuEChERS extraction method |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Buformin |
| キーワード |
|
|
主題 |
Postmortem distribution/redistribution |
| キーワード |
|
|
主題 |
Blood-brain barrier |
| キーワード |
|
|
主題 |
QuEChERS method |
| キーワード |
|
|
主題 |
Standard addition method |
| キーワード |
|
|
主題 |
LC-MS/MS |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Wurita, Amin
Hasegawa, Koutaro
Nozawa, Hideki
Yamagishi, Itaru
Minakata, Kayoko
Watanabe, Kanako
Suzuki, Osamu
|
| 書誌情報 |
Forensic Science International
巻 314,
p. 110376,
発行日 2020-09
|
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利 |
|
|
権利情報 |
Copyright 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利 |
|
|
権利情報 |
This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.forsciint.2020.110376. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
An autopsy for a suicidal case of a male in his 40s, who had died of poisoning due to ingestion of a large amount of buformin, was performed at our department. Buformin is biganide class agent used for patients of diabetes mellitus, which can occasionally cause severe lactic acidosis. The autopsy was performed about 10 days after his death, and the direct cause of his death was judged as asphyxia due to the aspiration of stomach contents into the airway. The nine body fluids and eight solid tissues specimens were dealt with for investigating postmortem distribution/redistribution of buformin in a whole body; femoral vein blood, right and left heart blood, pericardial fluid, urine, bile, stomach contents, small intestine contents, cerebrospinal fluid, the brain, lung, heart muscle, liver, spleen, kidney and skeletal muscle were examined. For extracting buformin from specimens, a modified QuEChERS method including dispersive solid-phase extraction was employed, followed by the analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS). Buformin in various kinds of human matrices were quantified by the standard addition method in this study, which can overcome the matrix effects and recovery rates without use of blank human matrices. All concentrations of buformin in specimens examined in this case were extremely higher than those of previously reported poisoning cases. The concentrations of buformin in left and right heart blood and femoral vein blood specimens of this case were 399, 216 and 261μg/mL, respectively; although the direct cause of his death was judged as asphyxia due to occlusion of airway with stomach contents, the vomiting was thought to be provoked by buformin poisoning. In this study, marked differences of buformin concentrations between brain tissue and cerebral spiral fluids, and other specimens were observed, which suggested that its distribution was influenced also by blood-brain-barrier. Although a number of buformin poisoning cases were published so far, they gave sporadic data on its concentrations and/or distribution in some limited human specimens. This study is the first to describe detailed distribution/redistribution of buformin in a whole human body quantified by using LC-MS/MS. |
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0379-0738 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1872-6283 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
32615395 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.forsciint.2020.110376 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
FSI-314-110376.pdf (1.3 MB)
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