Histological and Mechanical Information Based on Biochemical Alterations of Cardiovascular Diseases Using Scanning Acoustic Microscopy with Proteinases: A Novel Technique for Cardiovascular Research

Miura, Katsutoshi

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Histological and Mechanical Information Based on Biochemical Alterations of Cardiovascular Diseases Using Scanning Acoustic Microscopy with Proteinases: A Novel Technique for Cardiovascular Research

http://hdl.handle.net/10271/00003942

名前 / ファイル	ライセンス	アクション
Atheroscler OA-6-1000149.pdf (2.4 MB)

Item type

学術雑誌論文 / Journal Article(1)

公開日

2022-01-26

タイトル

Histological and Mechanical Information Based on Biochemical Alterations of Cardiovascular Diseases Using Scanning Acoustic Microscopy with Proteinases: A Novel Technique for Cardiovascular Research

言語

eng

キーワード

主題

Scanning acoustic microscopy

キーワード

主題

Speed of sound

キーワード

主題

Atherosclerosis

キーワード

主題

Aging

キーワード

主題

Myocardial infarction

キーワード

主題

Cardiac valve

キーワード

主題

Proteinase

資源タイプ

資源タイプ識別子

http://purl.org/coar/resource_type/c_6501

資源タイプ

journal article

著者

Miura, Katsutoshi

書誌情報

Atherosclerosis: Open Access

巻 6, 号 2, p. 1000149, 発行日 2021-02-24

出版者

OMICS International

権利

権利情報

© 2021 Miura K. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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内容記述タイプ

Abstract

内容記述

Scanning Acoustic Microscopy (SAM) simultaneously provides structural and mechanical information from a histological glass slide. It supplies objective evidence of damage or degeneration because the Speed-of-Sound (SOS) of each portion correlates with its stiffness. Moreover, comparing SOS values before and after proteinase treatment discloses the susceptibility of the breakdown. We used this method in a cardiovascular study on atherosclerosis, aging, myocardial infarction, cardiac valve dysfunction, and mechanical rupture. With aging, smooth muscles and elastic fibers of arteries and the aorta decrease in number, resulting in reduced SOS values. Although atherosclerosis makes tissues stiffer, mainly in the intima, the stiffness of the outside medial layer quantitatively decreases comparable to SOS values. Fibrosis after inflammation or infarction usually becomes stiffer than the original state. Older fibrosis shows more resistance to proteinase digestion, which indicates the additional modification in the proteins of the old fibrosis. Mechanical stress causes tissue fracture, such as rupture of the tendinous cord of the mitral valve. The ruptured portion shows an irregular array of fibers with loss of cells and myxoid degeneration where reduced SOS values and accumulation of some extracellular matrix components appear. Comparing Aortic Stenosis (AS) with regurgitation (AR) of the aortic valve, the fibrosa of both valves was stiffer than that of the normal valves. The fibrosa of AR is thinner but stiffer and more resistant to proteinase digestion than that of AS. Advanced glycation end-products are deposited more in the AR valve. Histological observation using SAM can reveal mechanical alterations based on biochemical abnormality.

著者版フラグ

出版タイプ

VoR

出版タイプResource

http://purl.org/coar/version/c_970fb48d4fbd8a85

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