| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-05-06 |
| タイトル |
|
|
タイトル |
Evaluation of MBT STAR-Cepha and MBT STAR-Carba kits for the detection of extended-spectrum β-lactamases and carbapenemase producing microorganisms using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry |
| キーワード |
|
|
主題 |
MBT STAR-Cepha kit |
| キーワード |
|
|
主題 |
MBT STAR-Carba kit |
| キーワード |
|
|
主題 |
extended-spectrum β-lactamase |
| キーワード |
|
|
主題 |
carbapenemase |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
MALDI-TOF MS based kits detect β-lactamase producer |
| 著者 |
Ota, Yusuke
Furuhashi, Kazuki
Hirai, Nachi
Ishikawa, Jinko
Nagura, Osanori
Yamanaka, Katsumasa
Maekawa, Masato
|
| 書誌情報 |
Journal of Microbiological Methods
巻 183,
p. 106166,
発行日 2021-04
|
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利 |
|
|
権利情報 |
Copyright 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license?http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利 |
|
|
権利情報 |
This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.mimet.2021.106166. |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Rapid and simple detection of extended-spectrum β-lactamase (ESBL) and carbapenemase is essential for antimicrobial treatment and infection control. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based MBT STAR-Cepha and MBT STAR-Carba kits have been developed with simplified MBT STAR-BL operations. However, the utility of these kits has not been fully examined in clinical microbiology laboratories. In this study, we evaluated the utility of MALDI-TOF MS-based MBT STAR-Cepha and MBT STAR-Carba kits to detect ESBL and carbapenemase-producing bacteria, and compared it with the conventional broth microdilution test and PCR amplification assay. We found that the MBT STAR-Cepha kit efficiently enabled to distinguish resistant strains of third-generation cephalosporin susceptibility phenotypes and non-SHV-type ESBL producers. In the receiver operating characteristic analysis, the area under the receiver operating characteristic curve (AUC) for detecting third-generation cephalosporin resistance using the MBT STAR-Cepha kit was 0.97-1.00, but the AUC for detecting ESBL producers was 0.64. In addition, we showed that the MBT STAR-Carba kit enable the accurate detection of antimicrobial resistance by IMP-type carbapenemase producers. The AUC for detecting carbapenemase producers was 1.00. The results suggested that the target bacterial strains, antimicrobial susceptibility phenotypes, and resistance genes were important for the utility of the MALDI-TOF MS-based MBT STAR-Cepha and MBT STAR-Carba kits in bacterial routine diagnostics. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0167-7012 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1872-8359 |
| PubMed番号 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
|
|
関連識別子 |
33600876 |
| 出版社DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.mimet.2021.106166 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
JMM-183-106166.pdf (1.7 MB)
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