| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-06-10 |
| タイトル |
|
|
タイトル |
Simple LC-MS/MS method using core-shell ODS microparticles for the simultaneous quantitation of edoxaban and its major metabolites in human plasma |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Edoxaban |
| キーワード |
|
|
主題 |
metabolites |
| キーワード |
|
|
主題 |
LC-MS/MS |
| キーワード |
|
|
主題 |
human plasma |
| キーワード |
|
|
主題 |
pharmacokinetics |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Ariizumi, Saki
Naito, Takafumi
Hoshikawa, Kohei
Akutsu, Shunta
Saotome, Masao
Maekawa, Yuichiro
Kawakami, Junichi
|
| 書誌情報 |
Journal of Chromatography B
巻 1146,
p. 122121,
発行日 2020-07
|
| 出版者 |
|
|
出版者 |
Elsevier |
| 権利 |
|
|
権利情報 |
Copyright?2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license?http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利 |
|
|
権利情報 |
This is the accepted manuscript version. The formal published version is available at https://doi.org/10.1016/j.jchromb.2020.122121. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Edoxaban is mainly enzymatically converted to a 4-carboxylic acid form (4CA-EDX) and an N-desmethyl form (ND-EDX) in humans. This study aimed to establish a simple liquid chromatography-tandem mass spectrometry method using core-shell octadecyl silica (ODS) microparticles for the simultaneous quantitation of edoxaban and its two major metabolites in human plasma. Analytes extracted from plasma specimens by a one-step deproteinization were separated using a 2.6-μm core-shell ODS microparticulate column and linear acetonitrile-ammonium acetate gradient elution at a flow rate of 0.25mL/min with a run time of 7 minutes. The mass spectrometer was operated in the positive ion multiple reaction monitoring mode. Plasma samples collected from 20 patients with atrial fibrillation were analyzed by the present method. The chromatograms of drug-free human plasma had no interfering peaks. The calibration curves of edoxaban, 4CA-EDX, and ND-EDX were linear over the concentration ranges of 1.25?160, 0.47?60, and 0.12?15ng/mL, respectively. Their pretreatment recoveries and matrix factors were 88.7?109.0% and 87.0?101.6%, respectively. The intra- and inter-day accuracy and imprecision values were 85.9?112.8% and within 13.3%, respectively. The plasma concentrations of edoxaban, 4CA-EDX, and ND-EDX in the patients had ranges of 17.8?102, 1.67?25.7, and 0.685?5.34ng/mL, respectively. All the analytes were measurable within their calibration curves. In conclusion, this validated method for the simultaneous determination of edoxaban and its major metabolites was successfully applied to plasma specimens obtained from patients with atrial fibrillation. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1570-0232 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1873-376X |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
32361632 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.jchromb.2020.122121 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
J Chromatogr B-1146-122121.pdf (1.1 MB)
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