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Protein Phosphatase Inhibitor-1 Augments a Protein Kinase A-Dependent Increase in the Ca2+ Loading of the Sarcoplasmic Reticulum Without Changing Its Ca2+ Release
http://hdl.handle.net/10271/1879
http://hdl.handle.net/10271/1879cebd03eb-33bb-40ef-8457-07de2c279bbb
名前 / ファイル | ライセンス | アクション |
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CircJ-73-1133.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-08-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Protein Phosphatase Inhibitor-1 Augments a Protein Kinase A-Dependent Increase in the Ca2+ Loading of the Sarcoplasmic Reticulum Without Changing Its Ca2+ Release | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Calyculin A | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Inhibitor-1 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Phospholamban | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Protein phosphatases | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sarcoplasmic reticulum | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
その他のタイトル | ||||||
その他のタイトル | 蛋白ホスファターゼ阻害因子-1は筋小胞体のCa+負荷をCa2+放出を変化させることなく蛋白キナーゼA依存性に増加させる | |||||
言語 | ja | |||||
著者 |
Kawashima, Hirotaka
× Kawashima, Hirotaka× Satoh, Hiroshi× Saotome, Masao× Urushida, Tsuyoshi× Katoh, Hideki× Hayashi, Hideharu |
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書誌情報 |
en : Circulation Journal 巻 73, 号 6, p. 1133-1140, 発行日 2010-05 |
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出版者 | ||||||
言語 | en | |||||
出版者 | THE JAPANESE CIRCULATION SOCIETY | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Copyright 2009 THE JAPANESE CIRCULATION SOCIETY | |||||
権利 | ||||||
言語 | ja | |||||
権利情報 | 本文データは学協会の許諾に基づきJ-STAGEから複製したものである | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: An increase in cytosolic protein phosphatases (PPs) de-phosphorylates phospholamban, decreasing the Ca2+ uptake of the sarcoplasmic reticulum (SR). The effects of PP inhibitors on cellular Ca2+ handling were investigated. Methods and Results: Twitch Ca2+ transients (CaTs) and cell shortening were measured in intact rat cardiac myocytes, and caffeine-induced Ca2+ transients (CaffCaTs) and Ca2+ sparks were studied in saponin-permeabilized cells. Calyculin A augmented isoproterenol-induced increases in CaTs and cell shortening without altering the diastolic [Ca2+]i and twitch [Ca2+]i decay. The protein kinase A catalytic subunit (PKAcat) increased the peak of CaffCaTs between 5 and 50 U/ml, and the addition of inhibitor-1 (I-1) augmented the increase. PKAcat increased Ca2+ spark frequency and the addition of I-1 increased it further. PKAcat at 50 U/ml amplified the peak and prolonged the duration of Ca2+ sparks, whereas the addition of I-1 did not alter them. An abrupt inhibition of SR Ca2+ uptake following exposure to PKAcat caused a gradual decrease in Ca2+ spark frequency, but the addition of I-1 did not accelerate the decline of Ca2+ spark frequency or CaffCaTs. Conclusions: Inhibition of PPs augmented the inotropic effect of isoproterenol. Specific inhibition of PP1 could stimulate the Ca2+ uptake of the SR with less significant effects on the Ca2+ release. | |||||
言語 | en | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13469843 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13474820 | |||||
出版社DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1253/circj.CJ-08-0871 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |