| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
Protein Phosphatase Inhibitor-1 Augments a Protein Kinase A-Dependent Increase in the Ca2+ Loading of the Sarcoplasmic Reticulum Without Changing Its Ca2+ Release |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Calyculin A |
| キーワード |
|
|
主題 |
Inhibitor-1 |
| キーワード |
|
|
主題 |
Phospholamban |
| キーワード |
|
|
主題 |
Protein phosphatases |
| キーワード |
|
|
主題 |
Sarcoplasmic reticulum |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
蛋白ホスファターゼ阻害因子-1は筋小胞体のCa+負荷をCa2+放出を変化させることなく蛋白キナーゼA依存性に増加させる |
| 著者 |
Kawashima, Hirotaka
Satoh, Hiroshi
Saotome, Masao
Urushida, Tsuyoshi
Katoh, Hideki
Hayashi, Hideharu
|
| 書誌情報 |
en : Circulation Journal
巻 73,
号 6,
p. 1133-1140,
発行日 2010-05
|
| 出版者 |
|
|
出版者 |
THE JAPANESE CIRCULATION SOCIETY |
|
言語 |
en |
| 権利 |
|
|
権利情報 |
Copyright 2009 THE JAPANESE CIRCULATION SOCIETY |
| 権利 |
|
|
権利情報 |
本文データは学協会の許諾に基づきJ-STAGEから複製したものである |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Background: An increase in cytosolic protein phosphatases (PPs) de-phosphorylates phospholamban, decreasing the Ca2+ uptake of the sarcoplasmic reticulum (SR). The effects of PP inhibitors on cellular Ca2+ handling were investigated. Methods and Results: Twitch Ca2+ transients (CaTs) and cell shortening were measured in intact rat cardiac myocytes, and caffeine-induced Ca2+ transients (CaffCaTs) and Ca2+ sparks were studied in saponin-permeabilized cells. Calyculin A augmented isoproterenol-induced increases in CaTs and cell shortening without altering the diastolic [Ca2+]i and twitch [Ca2+]i decay. The protein kinase A catalytic subunit (PKAcat) increased the peak of CaffCaTs between 5 and 50 U/ml, and the addition of inhibitor-1 (I-1) augmented the increase. PKAcat increased Ca2+ spark frequency and the addition of I-1 increased it further. PKAcat at 50 U/ml amplified the peak and prolonged the duration of Ca2+ sparks, whereas the addition of I-1 did not alter them. An abrupt inhibition of SR Ca2+ uptake following exposure to PKAcat caused a gradual decrease in Ca2+ spark frequency, but the addition of I-1 did not accelerate the decline of Ca2+ spark frequency or CaffCaTs. Conclusions: Inhibition of PPs augmented the inotropic effect of isoproterenol. Specific inhibition of PP1 could stimulate the Ca2+ uptake of the SR with less significant effects on the Ca2+ release. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13469843 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13474820 |
| 出版社DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1253/circj.CJ-08-0871 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
CircJ-73-1133.pdf (1.1 MB)
