{"_buckets": {"deposit": "1e234bec-5859-4cc5-8b77-ff753cd4a171"}, "_deposit": {"created_by": 4, "id": "2412", "owners": [4], "pid": {"revision_id": 0, "type": "depid", "value": "2412"}, "status": "published"}, "_oai": {"id": "oai:hama-med.repo.nii.ac.jp:00002412", "sets": ["11"]}, "author_link": [], "item_3_biblio_info_5": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1994-01-24", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "682", "bibliographicPageStart": "673", "bibliographicVolumeNumber": "35", "bibliographic_titles": [{"bibliographic_title": "Japanese Heart Journal"}]}]}, "item_3_description_30": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_3_description_9": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image processing. In guinea pig myocytes, Ca2+ waves were absent during the control perfusion period, but could be induced by the addition of strophanthidin (100μM) or sodium cyanide (NaCN: 2mM) to the perfusate. The [Ca2+]i increased from the control values of 69±5nM and 46±2nM, to 263±9 (p\u003c0.05 vs. control) nM and 225±20 (p\u003c0.05) nM, respectively, when cells exhibited Ca2+ waves. Although 13% (16 of 121) of the rat myocytes displayed Ca2+ waves during the control perfusion, the [Ca2+]i with Ca2+ waves (56±9nM) did not differ from [Ca2+]i in the absence of Ca2+ waves (54±3nM). Ca2+ waves were induced by the perfusion with a high Ca2+ solution (24.5μM) or NaCN (2μM), and [Ca2+]i increased from the control values of 67±11nM and 74±5nM, to 231±41 (p\u003c0.05 vs. control) nM and 266±64nM, respectively, when cells exhibited Ca2+ waves. The Ca2+ waves were abolished by the removal of extracellular Ca2+, or by the perfusion with ryanodine (10μM) or caffeine (20mM). In conclusion, it was shown that Ca2+ waves were due to oscillatory Ca2+ release and that the absolute value of [Ca2+]i is important for the appearance of Ca2+ waves in guinea pig and rat myocytes. However, some rat myocytes with a control [Ca2+]i level exhibited spontaneous Ca2+ waves during the control perfusion, showing a species difference in the susceptibility to oscillatory Ca2+ release from the sarcoplasmic reticulum.(Jpn Heart J 35: 673-682, 1994)", "subitem_description_type": "Abstract"}]}, "item_3_publisher_6": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "International Heart Journal Association"}]}, "item_3_relation_28": {"attribute_name": "出版社DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1536/ihj.35.673", "subitem_relation_type_select": "DOI"}}]}, "item_3_rights_7": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "by International Heart Journal Association"}]}, "item_3_select_2": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_select_item": "Journal Article", "subitem_select_language": "en"}]}, "item_3_source_id_19": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00214868", "subitem_source_identifier_type": "ISSN"}]}, "item_3_source_id_20": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1348673X", "subitem_source_identifier_type": "ISSN"}]}, "item_3_version_type_32": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hayashi, Hideharu"}]}, {"creatorNames": [{"creatorName": "Miyata, Haruo"}]}, {"creatorNames": [{"creatorName": "Terada, Hajime"}]}, {"creatorNames": [{"creatorName": "Satoh, Hiroshi"}]}, {"creatorNames": [{"creatorName": "Katoh, Hideki"}]}, {"creatorNames": [{"creatorName": "Nakamura, Takurou"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Akira"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-08-27"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "JHeartJ-35-673.pdf", "filesize": [{"value": "1.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1500000.0, "url": {"label": "JHeartJ-35-673.pdf", "url": "https://hama-med.repo.nii.ac.jp/record/2412/files/JHeartJ-35-673.pdf"}, "version_id": "f9dc2558-630a-44cd-9412-449f7a69d8cf"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Fluorescence digital processing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Oscillatory Ca2+ release", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sarcoplasmic reticulum", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Species difference", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fura-2", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ca2+ Waves and Intracellular Ca2+ Concentration in Guinea Pig and Rat Myocytes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Ca2+ Waves and Intracellular Ca2+ Concentration in Guinea Pig and Rat Myocytes", "subitem_title_language": "en"}]}, "item_type_id": "3", "owner": "4", "path": ["11"], "permalink_uri": "http://hdl.handle.net/10271/2477", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2013-08-27"}, "publish_date": "2013-08-27", "publish_status": "0", "recid": "2412", "relation": {}, "relation_version_is_last": true, "title": ["Ca2+ Waves and Intracellular Ca2+ Concentration in Guinea Pig and Rat Myocytes"], "weko_shared_id": -1}
Ca2+ Waves and Intracellular Ca2+ Concentration in Guinea Pig and Rat Myocytes
http://hdl.handle.net/10271/2477
http://hdl.handle.net/10271/2477