| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
Ca2+ Waves and Intracellular Ca2+ Concentration in Guinea Pig and Rat Myocytes |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Fluorescence digital processing |
| キーワード |
|
|
主題 |
Oscillatory Ca2+ release |
| キーワード |
|
|
主題 |
Sarcoplasmic reticulum |
| キーワード |
|
|
主題 |
Species difference |
| キーワード |
|
|
主題 |
Fura-2 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Hayashi, Hideharu
Miyata, Haruo
Terada, Hajime
Satoh, Hiroshi
Katoh, Hideki
Nakamura, Takurou
Kobayashi, Akira
|
| 書誌情報 |
Japanese Heart Journal
巻 35,
号 5,
p. 673-682,
発行日 1994-01-24
|
| 出版者 |
|
|
出版者 |
International Heart Journal Association |
| 権利 |
|
|
権利情報 |
by International Heart Journal Association |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We measured [Ca2+]i of guinea pig and rat myocytes with Ca2+ waves, using fura-2 fluorescence image processing. In guinea pig myocytes, Ca2+ waves were absent during the control perfusion period, but could be induced by the addition of strophanthidin (100μM) or sodium cyanide (NaCN: 2mM) to the perfusate. The [Ca2+]i increased from the control values of 69±5nM and 46±2nM, to 263±9 (p<0.05 vs. control) nM and 225±20 (p<0.05) nM, respectively, when cells exhibited Ca2+ waves. Although 13% (16 of 121) of the rat myocytes displayed Ca2+ waves during the control perfusion, the [Ca2+]i with Ca2+ waves (56±9nM) did not differ from [Ca2+]i in the absence of Ca2+ waves (54±3nM). Ca2+ waves were induced by the perfusion with a high Ca2+ solution (24.5μM) or NaCN (2μM), and [Ca2+]i increased from the control values of 67±11nM and 74±5nM, to 231±41 (p<0.05 vs. control) nM and 266±64nM, respectively, when cells exhibited Ca2+ waves. The Ca2+ waves were abolished by the removal of extracellular Ca2+, or by the perfusion with ryanodine (10μM) or caffeine (20mM). In conclusion, it was shown that Ca2+ waves were due to oscillatory Ca2+ release and that the absolute value of [Ca2+]i is important for the appearance of Ca2+ waves in guinea pig and rat myocytes. However, some rat myocytes with a control [Ca2+]i level exhibited spontaneous Ca2+ waves during the control perfusion, showing a species difference in the susceptibility to oscillatory Ca2+ release from the sarcoplasmic reticulum.(Jpn Heart J 35: 673-682, 1994) |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00214868 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1348673X |
| 出版社DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1536/ihj.35.673 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
JHeartJ-35-673.pdf (1.5 MB)
