| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
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|
タイトル |
Electrospray ionization tandem mass spectrometric determination of monomethylarsonic acid and dimethylarsinic acid after adduct formation with citric acid |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Monomethylarsonic acid |
| キーワード |
|
|
主題 |
Dimethylarsinic acid |
| キーワード |
|
|
主題 |
Tandem mass spectrometry |
| キーワード |
|
|
主題 |
Electrospray ionization |
| キーワード |
|
|
主題 |
Citric acid |
| キーワード |
|
|
主題 |
Adduct |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Minakata, Kayoko
Ohnishi, Kazunori
Nakamura, Satoki
Suzuki, Masako
Suzuki, Osamu
|
| 書誌情報 |
en : Journal of Chromatography B
巻 877,
号 25,
p. 2624-2629,
発行日 2009-09-01
|
| 出版者 |
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|
出版者 |
Elsevier |
|
言語 |
en |
| 権利 |
|
|
権利情報 |
Copyright 2009 Elsevier B.V. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Inorganic arsenic species are metabolized to monomethylarsonic acid (MMA V) and dimethylarsinic acid (DMA V) and excreted into urine. A simple, rapid and sensitive method has been developed using electrospray ionization tandem mass spectrometry (ESI-MS-MS) for the simultaneous determination of MMA V and DMA V. MMA V and DMA V in a sample were allowed to react with citric acid (CiA). Adduct compounds were extracted together with isoamyl alcohol (IAA). An aliquot (1-μL) of the IAA layer was directly injected into the ESI-MS-MS instrument, and was detected within 1 min. Quantification was done using selected reaction monitoring for MMA V and DMA V as follows. [MMAH + 2CiA - 3H2O]+ → [MMAH + CiA - 2H2O]+ [DMAH + CiA + MeOH - 2H2O]+ → [DMAH + MeOH - H2O]+ Where, MMAH and DMAH denote the protonated forms of MMA V and DMA V, and MeOH denotes methanol (carrier liquid in ESI-MS-MS). This method was validated for the analysis of urine samples. The limit of detection of As was 0.3 μgL-1 for MMA V and 0.6 μgL-1 for DMA V using 10 μL of sample solution. Results were obtained in <10 min with a linear calibration range of 3-100 μgL-1. Inorganic arsenic compounds (and other organic arsenic compounds) found in urine did not interfere with the detection of MMA V and DMA V. Concentrations of MMA V and DMA V in the reference urine SRM 2670a were estimated after partial purification, and those in urine of a patient treated with As2O3 were measured after dilution. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15700232 |
| 出版社DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.jchromb.2009.03.016 |
| 著者版フラグ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
JofC_B-877-2624.pdf (517.4 kB)
