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Expression of tumor suppressor FHIT is regulated by the LINC00173-SNAIL axis in human lung adenocarcinoma
http://hdl.handle.net/10271/0002000317
http://hdl.handle.net/10271/000200031752aea8a6-5631-4b96-9ab2-c7fb2c388f4e
| 名前 / ファイル | ライセンス | アクション |
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論文本文 (2.3 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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| 公開日 | 2025-02-10 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Expression of tumor suppressor FHIT is regulated by the LINC00173-SNAIL axis in human lung adenocarcinoma | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | lncRNA | |||||||||||
| キーワード | ||||||||||||
| 主題 | LINC00173 | |||||||||||
| キーワード | ||||||||||||
| 主題 | SNAIL | |||||||||||
| キーワード | ||||||||||||
| 主題 | FHIT | |||||||||||
| キーワード | ||||||||||||
| 主題 | lung adenocarcinoma | |||||||||||
| キーワード | ||||||||||||
| 主題 | tumor suppressor gene | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
| 資源タイプ | doctoral thesis | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| その他のタイトル | ||||||||||||
| その他のタイトル | ヒト肺腺がんにおいて腫瘍抑制遺伝子であるFHITの発現はLINC00173-SNAIL軸によって制御される | |||||||||||
| 著者 |
鈴木, 貴人
× 鈴木, 貴人
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| bibliographic_information |
en : International Journal of Molecular Sciences 巻 24, 号 23, p. 17011, 発行日 2023-11-30 |
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| 出版者 | ||||||||||||
| 出版者 | MDPI | |||||||||||
| 言語 | en | |||||||||||
| 権利 | ||||||||||||
| 言語 | en | |||||||||||
| 権利情報 | (C) 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Long non-coding RNAs (lncRNAs) play a critical role in a variety of human diseases such as cancer. Here, to elucidate a novel function of a lncRNA called LINC00173, we investigated its binding partner, target gene, and its regulatory mechanism in lung adenocarcinoma, including the A549 cell line and patients. In the A549 cell line, RNA immunoprecipitation (RIP) assays revealed that LINC00173 efficiently binds to SNAIL. RNA-seq and RT-qPCR analyses revealed that the expression of FHIT was decreased upon LINC00173 depletion, indicating that FHIT is a target gene of LINC00173. Overexpression of SNAIL suppressed and depletion of SNAIL increased the expression of FHIT, indicating that SNAIL negatively regulates FHIT. The downregulation of FHIT expression upon LINC00173 depletion was restored by additional SNAIL depletion, revealing a LINC00173-SNAILFHIT axis for FHIT regulation. Data from 501 patients with lung adenocarcinoma also support the existence of a LINC00173-SNAIL-FHIT axis, as FHIT expression correlated positively with LINC00173 (p=1.75×10 -6) and negatively with SNAIL (p=7.00×10 -5). Taken together, we propose that LINC00173 positively regulates FHIT gene expression by binding to SNAIL and inhibiting its function in human lung adenocarcinoma. Thus, this study sheds light on the LINC00173-SNAIL-FHIT axis, which may be a key mechanism for carcinogenesis and progression in human lung adenocarcinoma. | |||||||||||
| 言語 | en | |||||||||||
| 学位名 | ||||||||||||
| 学位名 | 博士（医学） | |||||||||||
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| 内容記述 | doctoral | |||||||||||
| item_5_description_14 | ||||||||||||
| 内容記述 | 医学系研究科 | |||||||||||
| item_5_degree_grantor_15 | ||||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||||
| 学位授与機関識別子 | 13802 | |||||||||||
| 学位授与機関名 | 浜松医科大学 | |||||||||||
| 学位授与機関名 | Hamamatsu University School of Medicine | |||||||||||
| 学位授与年月日 | ||||||||||||
| 学位授与年月日 | 2024-03-13 | |||||||||||
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| 学位授与番号 | 甲第969号 | |||||||||||
| EISSN | ||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||
| 収録物識別子 | 1422-0067 | |||||||||||
| item_5_source_id_23 | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA12038549 | |||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | PMID | |||||||||||
| 関連識別子 | 38069335 | |||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.3390/ijms242317011 | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
