| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
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タイトル |
EFFECTS OF HYDROGEN PEROXIDE ON STIMULATORY GUANINE NUCLEOTIDE-BINDING PROTEIN IN RAT HEART |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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主題 |
β-adrenergic receptor |
| キーワード |
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主題 |
Guanine nucleotide-binding protein |
| キーワード |
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主題 |
Adenylate cyclase |
| キーワード |
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主題 |
Oxygen free radicals |
| キーワード |
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主題 |
Hydrogen peroxide |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
増田, 尚道
Kaneko, Masanori
Rong, Bang Hong
Ikegaya, Takayoshi
Hayashi, Hideharu
Koobayashi, Akira
Yamazaki, Noboru
|
| 書誌情報 |
Japanese circulation journal
巻 57,
号 10,
p. 1007-1015,
発行日 1993-09-20
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| 出版者 |
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出版者 |
日本循環器学会 |
| 権利 |
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|
権利情報 |
Copyright 日本循環器学会 |
| 権利 |
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|
権利情報 |
本文データは学協会の許諾に基づきCiNiiから複製したものである |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
This study was undertaken to examine the effects of hydrogen peroxide on stimulatory guanine nucleotide-binding protein (Gs), and coupling in the β-aderenergic receptor - Gs - adenylate cyclase system in rat heart, in vitro. Cardiac membranes were preincubated with various concentrations (0.1, 1 , and 10 mM) of hydrogen peroxide at 30℃ for 5, 10, 30 and 60 min. Although the assay of β-adrenergic receptors involving [^3H]-dihydroaloprenolol ([^3H]-DHA) binding revealed that the maximal number of binding sites (B_<max>) was not altered, the dissociation constant (K_d) for [^3H]-DHA was increased in the presence of I mM and 10 mM hydrogen peroxide (control 0.68±0.16 nM, vs 1 mM H202 1.13±0.16, 10 mM H202 1.01±0. 12). Conversely, no significant changes in Gs activities were observed in hydrogen peroxide-treated groups. Adenylate cyclase activity (stimulated by forskolin) was significantly reduced by 10 mM hydrogen peroxide after a 5 min preincubation period (control 277.1±19.2 pmol cAMP/mg protein/min, H202 230.3±14.9). The amounts of cyclic AMP produced by the stimulation of membranes with GTP, GTP + (1)-isoproterenol, guanylimidodiphosphate (Gpp(NH)p) or Gpp(NH)p+(1)-isoproterenol were significantly lower in 10 mM hydrogen peroxide-treated groups than those in controls (GTP: control 57.6±5.6pmol cAMP/mg protein/min vs H_2O_2 46.4±6.9, GTP+(1)-isoproterenol: control 83.9±10.2 vs H_2O_2 67.7±10.3, Gpp(NH)p: control 77.5±8.8 vs H_2O_2 61.0±8.6. Gpp(NH)p+(1)-isoproterenol: control 105.0±13.1 vs H202 83.9±12.2, forskolin: control 223.2±13.8 vs H202 182.8±18.4). In the presence of hydrogen peroxide, the extents of the depression in cAMP production induced by GTP. GTP+isoproterenol, Gpp(NH)p, and Gpp(NH)p+ (1)-isoproterenol were similar to those induced by forskolin stimulation. These results indicate that hydrogen peroxide does not affect Gs activity or coupling in the β-receptor - Gs - adenylate cyclase system, but does depress cAMP production by inhibiting adenylate cyclase. |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00471828 |
| 出版社DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1253/jcj.57.1007 |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
CircJ57_10-1007.pdf (872.2 kB)
