| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-04-14 |
| タイトル |
|
|
タイトル |
Single strand conformation polymorphism (SSCP) パターンに及ぼす泳動条件の影響 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
polymerase chain reaction (PCR) |
| キーワード |
|
|
主題 |
single strand conformation polymorphism (SSCP) |
| キーワード |
|
|
主題 |
genetic mutation |
| キーワード |
|
|
主題 |
SDS, temperature |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
前川, 真人
須藤, 加代子
Dilip, Chandra Dey
小谷, 一夫
菅野, 剛史
|
| 書誌情報 |
生物物理化学
巻 38,
号 2,
p. 95-101,
発行日 1994-04-15
|
| 出版者 |
|
|
出版者 |
日本電気泳動学会 |
| 権利 |
|
|
権利情報 |
日本電気泳動学会 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We investigated the effects of electrophoretic gels, buffers or temperature on SSCP pattern by an automated electrophoretic system, Phast System. The electrophoresis on the 12.5% homogeneous gel gave the greatest difference in the mobility shift by genetic mutation. The SDS-containing buffer increased the resolution of each band as well as the sensitivity of detection in most cases. However, for certain mutated sequences, the native buffer was superior at detecting mutations. The mobility shift is also temperature dependent irrespective of native or SDS buffer condition. In any new mutation sequences, it is difficult to determine which is the better condition to detect the mutations, SDS or native buffer; however, the SDS-containing buffer may lead to a better sensitivity of detection. In conclusion, it is important to perform SSCP analysis under SDS buffer condition as well as native buffer condition in all cases. |
| ISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00319082 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13499785 |
| 出版社DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.2198/sbk.38.95 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Seibutubuturi38_2-95.pdf (4.8 MB)
