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SSCP法による癌関連遺伝子の解析
http://hdl.handle.net/10271/2440
http://hdl.handle.net/10271/2440f55f9ca9-e674-4c0e-b4db-da8ca52d0821
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Seibutubuturi45_1-5.pdf (2.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2013-08-27 | |||||||||
| タイトル | ||||||||||
| タイトル | SSCP法による癌関連遺伝子の解析 | |||||||||
| 言語 | ja | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 主題 | PCR-SSCP | |||||||||
| キーワード | ||||||||||
| 主題 | RT-PCR | |||||||||
| キーワード | ||||||||||
| 主題 | loss of heterozygosity | |||||||||
| キーワード | ||||||||||
| 主題 | methylation. | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
前川, 真人
× 前川, 真人
× 菅野, 康吉
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| 書誌情報 |
生物物理化学 巻 45, 号 1, p. 5-8, 発行日 2001-03-15 |
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| 出版者 | ||||||||||
| 出版者 | 日本電気泳動学会 | |||||||||
| 権利 | ||||||||||
| 権利情報 | 日本電気泳動学会 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Single-strand DNA conformation polymorphism (SSCP) analysis has been used to detect alterations in relatively short DNA fragments such as genetic polymorphisms, mutations, deletions, and so on. Here we introduce other applications of SSCP analysis, using its capacity of separating alleles. A sensitive method, designated as blunt-end SSCP analysis was developed for detecting loss of heterozygosity (LOH) in cancer tissues and urine samples. The method is fluorescence-based SSCP analysis, using blunt-end DNA fragments and applied for detecting an LOH of the p53 gene. The combination of reverse transcribed-PCR and fluorescence-based SSCP analysis is proposed for the quantitative determination of ratio of mRNA molecules with homologous sequences. The procedure is applicable to a determination of expression levels of genes such as lactate dehydrogenase subunits and cyclooxygenases 1 and 2. The combination of bisulfite treatment and PCR-SSCP analysis is proposed for quantitative methylation assay. This analytic procedure can be applied to the rapid identification of methylation status in multiple samples, quantification of methylated vs. unmethylated sequences and detection of methylation heterogeneity in the amplified DNA fragments. PCR-SSCP analysis is advantageous in simple procedure with relatively high sensitivity and is applicable to any other fields. | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 00319082 | |||||||||
| EISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 13499785 | |||||||||
| 出版社DOI | ||||||||||
| 関連タイプ | isIdenticalTo | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.2198/sbk.45.5 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
