| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
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|
タイトル |
EFFECTS OF PHOSPHOLIPASE C ON ACTION POTENTIALS AND INTRACELLULAR Ca2+ CONCENTRATION IN GUINEA PIG HEART |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Phospholipase C |
| キーワード |
|
|
主題 |
Fura-2 |
| キーワード |
|
|
主題 |
Intracellular Ca2+ concentration |
| キーワード |
|
|
主題 |
Delayed afterdepolarization |
| キーワード |
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|
主題 |
Ca2+ overload |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Hayashi, Hideharu
Miyata, Haruo
Terada, Hajime
Noda, Naohisa
Satoh, Hiroshi
Kobayashi, Akira
Yamazaki, Noboru
|
| 書誌情報 |
Japanese Circulation Journal
巻 57,
号 4,
p. 344-352,
発行日 1992-03-12
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| 出版者 |
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出版者 |
日本循環器学会 |
| 権利 |
|
|
権利情報 |
Copyright日本循環器学会 |
| 権利 |
|
|
権利情報 |
本文データは学協会の許諾に基づきJournal Archiveから複製したものである |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
To clarify the role of phospholipase C (PLC) in arrythmias and cell injury during myocardial ischemia/reperfusion, we studied its effects on electrophysiology and [Ca2+]i in guinea pig hearts. After exposure to PLC (1 and 2 U/ml), the action potential durations of right ventricular papillary muscles were decreased. Delayed afterdepolarizations were observed in all of the preparations, and some developed into triggered activities. Developed tension decreased after an initial increase for the first 5 min, while resting tension increased consistently. The effects of PLC (0.02, 0.1, and 0.2 U/ml) on [Ca2+]i of ventricular myocytes were measured using fura-2 fluorescence. The ratio of rod-shaped cells to all cells decreased in a time- and a concentration-dependent manner. Perfusion with 0.1 U/ml PLC elevated [Ca2+]i from 56±5 nM to 245 ±47 nM before cell rounding, and to 1167±172 nM after cell rounding, suggesting that PLC causes Ca2+ overload. In conclusion, activation of PLC may play a role in arrhythmias and cell injury during ischemia/reperfusion. The increase in [Ca2+]i during ischemia/reperfusion may activate phospholipase, which would further increase [Ca2+]i to form a vicious cycle. |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00471828 |
| EISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13474839 |
| 出版社DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.1253/jcj.57.344 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
JCircJ-57-344.pdf (1.1 MB)
