| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-10-05 |
| タイトル |
|
|
タイトル |
Development of tissue factor-targeted liposomes for effective drug delivery to stroma-rich tumors |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Antibody |
| キーワード |
|
|
主題 |
liposome |
| キーワード |
|
|
主題 |
pancreatic tumor |
| キーワード |
|
|
主題 |
targeting |
| キーワード |
|
|
主題 |
tissue factor |
| キーワード |
|
|
主題 |
tumor stroma |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Shimizu, Kosuke
Takeuchi, Yoshihito
Otsuka, Kazuma
Mori, Tomoya
Narita, Yudai
Takasugi, Shohei
Magata, Yasuhiro
Matsumura, Yasuhiro
Oku, Naoto
|
| 書誌情報 |
en : Journal of Controlled Release
巻 323,
p. 519-529,
発行日 2020-07-10
|
| 出版者 |
|
|
出版者 |
Elsevier |
|
言語 |
en |
| 権利 |
|
|
権利情報 |
Copyright 2020 Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| 権利 |
|
|
権利情報 |
This is the accepted manuscript version. The formal published version is available at "https://doi.org/10.1016/j.jconrel.2020.04.043". |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Tissue factor (TF), which is well known as a trigger molecule of extrinsic coagulation, is found in not only tumor cells but also in stromal cells in tumor tissues. Thus, TF is a candidate molecule to potentially enable targeting of both tumor cells and stromal cells for anti-cancer drug delivery. Herein, we prepared liposomes conjugated with the Fab’ fragment of anti-TF antibody (TF Ab- Lip) and evaluated the capability for drug delivery to stroma-rich tumors for realizing a whole tumor tissue-targetable strategy. When the targetability of TF Ab-Lip to TF-expressing KLN205 squamous tumor cells and NIH3T3 fibroblast cells were examined, TF Ab-Lip was significantly taken up into both cells compared with non-targeted liposomes. Corresponding to this result, doxorubicin-encapsulated TF Ab-Lip (TF Ab-LipDOX) showed potent cytotoxicity against KLN205 cells. In vivo experiments using KLN205 solid tumor-bearing mice indicated that TF Ab-Lip became highly accumulated and distributed widely in not only the tumor cell region but also in the stromal one in the tumor. Treatment with TF Ab-LipDOX significantly suppressed the growth of KLN205 solid tumors. Furthermore, TF Ab-Lip targetable both mouse and human TF (mhTF Ab-Lip) became distributed throughout stroma-rich human pancreatic BxPC3 tumors and the treatment of the BxPC3 tumor-bearing mice with mhTF Ab-LipDOX showed highest tumor suppressive effect. These data suggest that TF Ab-Lip could achieve effective accumulation for stroma-rich tumor treatment. |
| 注記 |
|
|
内容記述 |
＜参照＞出版社サイト：https://doi.org/10.1016/j.jconrel.2020.04.043 |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0168-3659 |
| EISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1873-4995 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
32360306 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1016/j.jconrel.2020.04.043 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 出版社版 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1016/j.jconrel.2020.04.043 |
JCR-323-519.pdf (9.7 MB)
.png)
