| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-03-15 |
| タイトル |
|
|
タイトル |
Microscopic observation of chemical modification in sections using scanning acoustic microscopy |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
ageing |
| キーワード |
|
|
主題 |
elasticity |
| キーワード |
|
|
主題 |
formalin fixation |
| キーワード |
|
|
主題 |
histochemistry |
| キーワード |
|
|
主題 |
histopathology |
| キーワード |
|
|
主題 |
imaging analysis |
| キーワード |
|
|
主題 |
pepsin |
| キーワード |
|
|
主題 |
periodic acid-Schiff reaction |
| キーワード |
|
|
主題 |
ultrasound |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
Ultrasound detection of tissue modification |
| 著者 |
Miura, Katsutoshi
Egawa, Yuki
Moriki, Toshiaki
Mineta, Hiroyuki
Harada, Hidekazu
Baba, Satoshi
Yamamoto, Seiji
|
| 書誌情報 |
Pathology International
巻 65,
号 7,
p. 355-366,
発行日 2015-07
|
| 出版者 |
|
|
出版者 |
John Wiley and Sons |
| 権利 |
|
|
権利情報 |
This is the peer reviewed version of the following article: "Microscopic observation of chemical modification in sections using scanning acoustic microscopy", Pathology International; 65(7): p355-366, 2015, which has been published in final form at https://doi.org/10.1111/pin.12288. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
A scanning acoustic microscope (SAM) calculates the speed of sound (SOS) through tissues and plots the data on the screen to form images. Hard tissues result in greater SOS; based on these differences in tissue properties regarding SOS, SAM can provide data on tissue elasticity. The present study evaluated whether tissue modifications, such as formalin fixation, periodic acid-Schiff (PAS) reactions and protein degradation, changed the acoustic properties of the tissues and whether SAM could be a useful tool for following chemical changes in sections. The fixation process was observable by the increased SOS. During the PAS reaction, the glycosylation of tissues was characterized by an increased SOS. Mucous or glycogen distribution was visualised and was found to be statistically comparable among lesions and states. Protease digestion by pepsin led to a decreased SOS. Tissue sensitivity to proteases varied due to the stage, cause and duration of inflammation or ageing. Changes in acoustic properties were more sensitive than those in optical histology. SAM facilitates the visualisation of the time course or distribution of chemical modifications in tissue sections, thus aiding their comparison among tissues. SAM may be an effective tool for studying changes such as protein cross-linkage, tissue repair and ageing. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1320-5463 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1440-1827 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
25824722 |
| 医中誌論文ID |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
ICHUSHI |
|
|
関連識別子 |
2016231362 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1111/pin.12288 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
Pathol Int-65-355.pdf (2.4 MB)
