| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-10-12 |
| タイトル |
|
|
タイトル |
Plgrkt-deficient female mice caused by a 1-bp deletion of exon4 can maintain colonies through sufficient lactation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Plg-RKT deficiency |
| キーワード |
|
|
主題 |
lactation failure |
| キーワード |
|
|
主題 |
genome-editing |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
Intact lactation in Plgrkt-deficient female mice |
| 著者 |
Iwaki, Takayuki
Tomonari, Yuki
Umemura, Kazuo
|
| 書誌情報 |
Journal of Dairy Research
巻 89,
号 2,
p. 169-172,
発行日 2022-05
|
| 出版者 |
|
|
出版者 |
Cambridge University Press |
| 権利 |
|
|
権利情報 |
Copyright The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation |
| 権利 |
|
|
権利情報 |
"This article has been published in a revised form in ""Journal of Dairy Research"" https://doi.org/10.1017/S0022029922000292. This version is free to view and download for private research and study only. Not for re-distribution or re-use." |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Plasminogen (Pg) activation on the cell surfaces is an important for various (patho) physiologic condition. Plg-RKT is a cell membrane protein that binds to Pg and promotes the activation on the cell surface by Pg activators. To evaluate the role of Plg-RKT in atherosclerosis, Plgrkt gene in Ldlr-/-/Apobec1-/- was modified using in vivo CRISPR/Cas9. Synthetic RNA for Plgrkt and Cas9 complex was electroporated into the fertilized eggs in the oviducts. Plgrkt deficient mice caused by a 1-bp deletion was established. In contrast to Plgrkt-/- mice developed by conventional method, this newly developed mice did not represent lactation failure and could maintain the pups until weanling. The major obvious difference between these lines is the area of gene modification. The conventionally developed mouse possesses about 10 kb deletion of Plgrkt, which might relate to the lactation failure. Lactation failure is one of the lethal phenotypes in mammals, and analyses of causative genes are especially important for dairy industries. Further genome-wide analyses with both Plgrkt-/- mice will be helpful to find out the uncertain causative genes for lactation failure. |
| 注記 |
|
|
内容記述 |
雑誌掲載タイトル：Successful lactation in Plgrkt-deficient female mice caused by a 1-bp deletion of exon4 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0022-0299 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1469-7629 |
| NII書誌ID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00696465 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
35383548 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1017/S0022029922000292 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
J. Dairy Res-89-169.pdf (569.3 kB)
.png)
