| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
VON-WILLEBRAND-FACTOR |
| キーワード |
|
|
主題 |
EUGLOBULIN CLOT LYSIS |
| キーワード |
|
|
主題 |
WEIBEL-PALADE BODIES |
| キーワード |
|
|
主題 |
PROTEIN-KINASE-C |
| キーワード |
|
|
主題 |
T-PA |
| キーワード |
|
|
主題 |
MYOCARDIAL-INFARCTION |
| キーワード |
|
|
主題 |
PLASMA-LEVELS |
| キーワード |
|
|
主題 |
ACUTE RELEASE |
| キーワード |
|
|
主題 |
EXOCYTOSIS |
| キーワード |
|
|
主題 |
FIBRINOLYSIS |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Suzuki, Yuko
Mogami, Hideo
Ihara, Hayato
Urano, Tetsumei
|
| 書誌情報 |
en : Blood
巻 113,
号 2,
p. 470-478,
発行日 2009-01-08
|
| 出版者 |
|
|
出版者 |
American Society of Hematology |
|
言語 |
en |
| 権利 |
|
|
権利情報 |
Copyright 2009 by American Society of Hematology |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We analyzed the secretory dynamics of tissue plasminogen activator (tPA) in EA.hy926 cells, an established vascular endothelial cell (VEC) line producing GFP-tagged tPA, using total internal reflection fluorescence (TIR-F) microscopy. tPA-GFP was detected in small granules in EA.hy926 cells, the distribution of which was indistinguishable from intrinsically expressed tPA. Its secretory dynamics were unique, with prolonged (>5min.) retention of the tPA-GFP on the cell surface, appearing as fluorescent spots in two-thirds of the exocytosis events. The rapid disappearance (mostly by 250ms) of a domain-deletion mutant of tPA-GFP possessing only the signal peptide and catalytic domain indicates that the amino-terminal heavy chain of tPA-GFP is essential for binding to the membrane surface. The addition of PAI-1 dose-dependently facilitated the dissociation of membrane-retained tPA and increased the amounts of tPA-PAI-1 high molecular weight complexes in the medium. Accordingly, suppression of PAI-1 synthesis in EA.hy926 cells by siRNA prolonged the dissociation of tPA-GFP, whereas a catalytically inactive mutant of tPA-GFP not forming complexes with PAI-1 remained on the membrane even after PAI-1 treatment. Our results provide new insights into the relationship between exocytosed, membrane-retained tPA and PAI-1, which would modulate cell surface-associated fibrinolytic potential. |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00064971 |
| EISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
15280020 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1182/blood-2008-03-144279 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
blood-113-470.pdf (533.5 kB)
