| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
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|
タイトル |
Release of Ecdysteroid-Phosphates from Egg Yolk Granules and Their Dephosphorylation during Early Embryonic Development in Silkworm, Bombyx mori |
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言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
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|
主題 |
ecdysteroids |
| キーワード |
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|
主題 |
phosphatase |
| キーワード |
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|
主題 |
V-ATPase |
| キーワード |
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|
主題 |
vitellin |
| キーワード |
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|
主題 |
embryonic development |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Yamada, Ryouichi
Yamahama, Yumi
Sonobe, Haruyuki
|
| 書誌情報 |
en : ZOOLOGICAL SCIENCE
巻 22,
号 2,
p. 187-198,
発行日 2005
|
| 出版者 |
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出版者 |
Zoological Society of Japan |
|
言語 |
en |
| 権利 |
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|
権利情報 |
Copyright 2005 Zoological Society of Japan |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Newly laid eggs of many insect species store maternal ecdysteroids as physiologically inactive phosphoric esters. In the silkworm Bombyx mori, we previously reported the presence of a specific enzyme, called ecdysteroid-phosphate phosphatase (EPPase), which catalyzes the dephosphorylation of ecdysteroid-phosphates to increase the amount of free ecdysteroids during early embryonic development. In this study, we demonstrated that (1) EPPase is found in the cytosol of yolk cells, (2) ecdysteroid-phosphates are localized in yolk granules, being bound to the yolk protein vitellin (Vn), and (3) Vn-bound ecdysteroid-phosphates are scarcely hydrolyzed by EPPase, although free ecdysteroid-phosphates are completely hydrolyzed by EPPase. Thus, we investigated the mechanism by which ecdysteroid-phosphates dissociate from the Vn-ecdysteroid-phosphate complex, and indicated that the acidification of yolk granules causes the dissociation of ecdysteroid-phosphates from the Vn-ecdysteroid-phosphate complex and thereby ecdysteroid-phosphates are released from yolk granules into the cytosol. Indeed, the presence of vacuolar-type proton-translocating ATPase in the membrane fraction of yolk granules was also verified by Western blot analysis. Our experiments revealed that Vn functions as a reservoir of maternal ovarian ecdysteroid-phosphates as well as a nutritional source during embryonic development. This is the first report showing the biochemical mechanism by which maternal Vn-bound ecdysteroid-phosphates function during early embryonic development. |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
02890003 |
| 出版社DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.2108/zsj.22.187 |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ZOOLOGICAL SCIENCE-22-187.pdf (1.3 MB)
