| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-27 |
| タイトル |
|
|
タイトル |
低温オーブントラッピングキャピラリーガスクロマトグラフィーによるヒト血中全身麻酔薬の高感度分析 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| その他のタイトル |
|
|
その他のタイトル |
SENSITIVE DETERMINATION OF GENERAL ANAESTHETICS IN HUMAN WHOLE BLOOD BY CAPILLARY GAS CHROMATOGRAPHY WITH CRYOGENIC OVEN TRAPPING |
| 著者 |
小嶋, 高志
石井, 晃
勝又, 義直
鈴木, 加奈子
妹尾, 洋
鈴木, 修
熊澤, 武志
|
| 書誌情報 |
ja : 法中毒
en : Japanese journal of forensic toxicology
巻 18,
号 2,
p. 134-135,
発行日 2000-05-15
|
| 出版者 |
|
|
出版者 |
日本法中毒学会 |
|
言語 |
ja |
| 権利 |
|
|
権利情報 |
Copyright 日本法中毒学会 |
| 権利 |
|
|
権利情報 |
本文データは日本法中毒学会の許諾のもと掲載しています。 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Halothane, enflurane, isoflurane and sevoflurane are widely used as general anaesthetics. Their toxic concentrations are only 2 - 4 times higher compared to their therapeutic concentrations, and their abusers are frequently reported. Thus sensitive determinations for these anaesthetics must be important. In this presentation, we have applied cryogenic oven trapping for determining above four general anaesthetics in human whole blood samples. A 0.5-ml human whole blood with or without anaesthetics was added to a 7-ml vial containing 0.48 ml of distilled water and 0.02 ml of 10 % Triton X-100 solution (containing 5 μg internal standard), and the mixture was heated at 55°C for 15 min. A 10-ml headspace vapor was introduced into a GC capillary column in the splitless mode at -40°C oven temperature, that was programmed up to 250°C for GC analysis with flame ionization detection. All four peaks of the anaesthetics were clearly separated, and the backgrounds were very clean. Their extraction efficiencies were 8.3 - 15.3 %. The calibration curves showed good linearity in the range of 0.5 - 20 μg/ml and the determination limits were 20 100 ng/ml, which were comparable to previous GC-mass spectrometric (MS) and GC-Fourier transform infrared spectroscopy (FTIR) methods. Our present method should be useful for forensic toxicology; it can be also rapid monitoring for their anaesthetics in the human blood. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
09159606 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
FT-18-134.pdf (111.8 kB)
