| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-08-20 |
| タイトル |
|
|
タイトル |
Development of a novel T cell-oriented vaccine using CTL/Th-hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
CTL/Th-hybrid epitope long peptide |
| キーワード |
|
|
主題 |
Cross-presentation |
| キーワード |
|
|
主題 |
Intracellular bacteria |
| キーワード |
|
|
主題 |
Microparticle |
| キーワード |
|
|
主題 |
PLGA |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Tanaka, Kazuki
Enomoto, Noriyuki
Uehara, Masahiro
Furuhashi, Kazuki
Sakurai, Shogo
Yasui, Hideki
Karayama, Masato
Hozumi, Hironao
Suzuki, Yuzo
Fujisawa, Tomoyuki
Inui, Naoki
Nakamura, Yutaro
Nagata, Toshi
Suda, Takafumi
|
| 書誌情報 |
Microbiology and immunology
巻 64,
号 10,
p. 666-678,
発行日 2020-10
|
| 出版者 |
|
|
出版者 |
John Wiley and Sons |
| 権利 |
|
|
権利情報 |
"This is the peer reviewed version of the following article: ""Development of a novel T cell-oriented vaccine using CTL/Th-hybrid epitope long peptide and biodegradable microparticles, against an intracellular bacterium, Microbiology and immunology, 64(10): 666-678, (2020)"", which has been published in final form at https://doi.org/10.1111/1348-0421.12836. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited." |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Antigen-specific CD8+ T-lymphocytes (cytotoxic T-lymphocytes: CTL), as well as CD4+ T-lymphocytes (helper T-lymphocytes: Th), simultaneously play an important role in elimination of intracellular bacteria such as Mycobacterium tuberculosis and Listeria monocytogenes. Administration of T-cell epitope short peptide needs large numbers of peptides for effective vaccination due to its easily degradable nature in vivo. In this respect, biocompatible and biodegradable microparticles combined with CTL/Th-hybrid epitope long peptide (long peptide) have been used to diminish the loaded peptide degradation. The aim of this study is to develop a novel T cell-oriented vaccine against intracellular bacteria that is composed of long peptide and poly (lactic-co-glycolic acid) (PLGA) microparticles. Mouse bone marrow-derived dendritic cells (BMDCs) were loaded with L. monocytogenes listeriolysin O (LLO)-derived or ovalbumin (OVA)-derived long peptide/PLGA or other comparative antigens. The antigen-loaded BMDCs were subcutaneously injected into flank of mice twice, and then, the spleens were collected and lymphocyte proliferation and interferon-γ production were evaluated. The median diameter of PLGA spheres was 1.38 μm. Both LLO- and OVA-long peptide/PLGA showed significantly more robust CTL and Th proliferations with higher interferon-γ production than the long peptide alone or CTL and Th short peptides/PLGA vaccination. Furthermore, the LLO-long peptide/PLGA vaccination showed significantly lower bacterial burden in spleens compared with the long peptide alone or the CTL and Th short peptides/PLGA vaccination after the challenge of lethal amounts of L. monocytogenes. These results suggest that the novel vaccine taking advantages of CTL/Th-hybrid epitope long peptide and PLGA microparticle is effective for protection against intracellular bacteria. |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0385-5600 |
| EISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1348-0421 |
| PubMed番号 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
PMID |
|
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関連識別子 |
32786043 |
| 出版社DOI |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1111/1348-0421.12836 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
Microbiol immunol.-64-666.pdf (1.7 MB)
