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Acoustic histology with specific dyes and antibodies
http://hdl.handle.net/10271/00003940
http://hdl.handle.net/10271/00003940052967bc-d74e-40f9-bd1d-de0c76f2e52e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Ultrasound Med Biol-45-3279.pdf (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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| 公開日 | 2022-01-26 | |||||||||
| タイトル | ||||||||||
| タイトル | Acoustic histology with specific dyes and antibodies | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | Scanning acoustic microscopy | |||||||||
| キーワード | ||||||||||
| 主題 | histology | |||||||||
| キーワード | ||||||||||
| 主題 | cytology | |||||||||
| キーワード | ||||||||||
| 主題 | attenuation-of-sound | |||||||||
| キーワード | ||||||||||
| 主題 | hematoxylin | |||||||||
| キーワード | ||||||||||
| 主題 | special staining | |||||||||
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| 主題 | immunostaining | |||||||||
| キーワード | ||||||||||
| 主題 | collagenase | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | journal article | |||||||||
| 著者 |
Miura, Katsutoshi
× Miura, Katsutoshi
× Yamashita, Kanna
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| 書誌情報 |
Ultrasound in Medicine & Biology 巻 45, 号 12, p. 3279-3289, 発行日 2019-12 |
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| 出版者 | ||||||||||
| 出版者 | Elsevier | |||||||||
| 権利 | ||||||||||
| 権利情報 | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||
| 権利 | ||||||||||
| 権利情報 | This is the accepted manuscript version. The formal published version is available at "https://doi.org/10.1016/j.ultrasmedbio.2019.09.003". | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The present study aims to identify specific staining methods for acoustic histology. We compared attenuation-of-sound (AOS) images from scanning acoustic microscopy (SAM) with light microscopy (LM) images. Ethanol-fixed tissue or cytology samples and formalin-fixed surgical or autopsy specimens were examined. Nuclei, collagen, elastic fibers, and polysaccharides and various antigens, including cell surface, cytoplasmic, nuclear, and stromal substances, were observed. Samples with different fixation methods were used. Hematoxylin staining had significantly higher AOS values in accordance with staining duration. Specific staining for collagen, elastic fibers, and polysaccharides increased the AOS values of the specific substance. . Using diaminobenzidine tetrahydrochloride in NiCl2 solution as a substrate for horseradish peroxidase increased the AOS values to those suitable for acoustic immunostaining. Collagenase digestion following collagen staining decreased AOS values, reflecting collagen density and distribution. Staining with specific dyes or acoustic immunostaining enabled the histological localization of specific substances by SAM, similar to LM. | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0301-5629 | |||||||||
| EISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 1879-291X | |||||||||
| PubMed番号 | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | PMID | |||||||||
| 関連識別子 | 31563478 | |||||||||
| 出版社DOI | ||||||||||
| 関連タイプ | isVersionOf | |||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | 10.1016/j.ultrasmedbio.2019.09.003 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
